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Updated: Jul 10, 2026
11:06
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
Published on: June 30, 2018
Localization of proteins and organelles using fluorescence microscopy
Jean-Claude Farre1, Kanae Shirahama-Noda, Lan Zhang
1Section of Molecular Biology, Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 24, 2007
Summary
This study details methods for protein and organelle localization in Pichia pastoris using plasmids and immunofluorescence. Protocols are provided for visualizing subcellular components and their co-localization.
Area of Science:
- Cell Biology
- Microbiology
- Molecular Biology
Background:
- Accurate protein and organelle localization is crucial for understanding cellular function in Pichia pastoris.
- Existing methods may require optimization for specific applications in yeast research.
Purpose of the Study:
- To describe established and modified methods for protein and organelle localization in Pichia pastoris.
- To provide practical protocols for researchers working with this yeast model system.
Main Methods:
- Utilization of a series of specialized plasmids for protein expression and tagging.
- Application of a modified immunofluorescence protocol for precise localization studies.
- Employing vital stains for direct labeling of subcellular organelles.
Main Results:
- Detailed protocols for protein and organelle localization are presented.
- The described immunofluorescence protocol enables accurate localization and co-localization analysis.
- Vital staining methods allow for visualization of various subcellular compartments.
Conclusions:
- The chapter provides a comprehensive guide to protein and organelle localization techniques in Pichia pastoris.
- These methods facilitate detailed investigations into yeast cell biology and function.
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