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Updated: Jul 10, 2026

Optimized Production and Analysis of Recombinant Protein-Filled Vesicles from E. coli
Published on: June 30, 2023
Periplasmic targeting of recombinant proteins in Escherichia coli
Filipe J Mergulhão1, Gabriel A Monteiro
1LEPAE, Faculty of Engineering of the University of Porto, Chemical Engineering Department, Porto, Portugal.
Abstract:
Targeting recombinant protein production to the periplasmic space of Escherichia coli presents several advantages over cytoplasmic production in inclusion bodies and at the same time overcomes the low productivity problem often associated with culture medium secretion. This chapter presents a strategy for periplasmic production of recombinant proteins fused to synthetic Z domains derived from staphylococcal protein A. Expression, purification, and monitoring strategies are discussed using green fluorescent protein and human proinsulin as model proteins.
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