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Related Experiment Video

Updated: Jul 10, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System

Published on: July 17, 2021

Recombinant baculovirus isolation.

Linda A King¹, Richard Hitchman, Robert D Possee

¹School of Biological and Molecular Sciences, Oxford Brookes University, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)

|October 24, 2007

Summary

This study details methods for creating recombinant baculovirus expression vectors, focusing on insect cell transfection and recombinant virus isolation techniques. It covers essential steps like plaque assays and amplification for efficient gene expression vector development.

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Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

Recombinant baculovirus expression vectors are crucial tools in molecular biology.
Existing methods for generating these vectors often involve complex steps like insect cell transfection and plaque assays.

Purpose of the Study:

To provide an overview of the historical development of recombinant baculovirus isolation systems.
To detail practical methods for creating and isolating recombinant baculoviruses for gene expression.

Main Methods:

Insect cell transfection with viral DNA or DNA and transfer vectors.
Homologous recombination for gene transfer into the baculovirus genome.
Plaque assays for separating parental and recombinant viruses.
Small-scale amplification and titration of recombinant viruses.
Bacterial transformation and bacmid DNA isolation for the Bac-to-Bac system.

Main Results:

The chapter outlines various methods for generating recombinant baculoviruses.
It emphasizes techniques for efficient isolation and amplification of desired recombinant viruses.
Specific protocols for the Bac-to-Bac system are detailed, including bacmid DNA preparation.

Conclusions:

Efficient methods for producing and isolating recombinant baculoviruses are essential for gene expression studies.
The described techniques facilitate the development of reliable baculovirus expression systems.
Advancements in these methods have improved the efficiency of creating recombinant baculoviruses.