Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of ANCHOR viruses to validate radiative, physical and chemical decontamination systems in operational conditions.

The Journal of hospital infection·2026
Same author

SG33, a Vaccine Strain of Myxoma Virus with Oncolytic Potential, Exploits Macropinocytosis and Clathrin-Mediated Endocytosis for Entry into Pancreatic Cancer Cells.

Human gene therapy·2026
Same author

Tracking replicating HPV genomes in proliferating keratinocytes.

mBio·2025
Same author

LAVR-289, a broad-spectrum antiviral, protects immunosuppressed Syrian hamsters against lethal adenovirus challenge.

Antiviral research·2025
Same author

Real-time genome imaging of host interactions in adeno-associated virus genome release.

iScience·2025
Same author

Antiviral Activity of the Acyclic Nucleoside Phosphonate Prodrug LAVR-289 against Poxviruses and African Swine Fever Virus.

ACS infectious diseases·2025

Related Experiment Video

Updated: Dec 31, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

21.9K

Tracing Baculovirus AcMNPV Infection Using a Real-Time Method Based on ANCHORTM DNA Labeling Technology.

Aurélie Hinsberger1, Benoît Graillot1, Christine Blachère Lopez1,2

  • 1LGEI, IMT Mines Alès, Institut Mines-Télécom et Université de Montpellier Sud de France, 6 Avenue de Clavières, 30100 Alès, France.

Viruses
|January 8, 2020
PubMed
Summary

Researchers developed autofluorescent baculoviruses using ANCHOR™ technology to track viral infection dynamics. This breakthrough allows direct monitoring of individual virus genomes and infection progression in living cells and tissues.

Keywords:
baculovirus infectionfluorescence labellingreal-time imaging

More Related Videos

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

12.5K
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2
10:55

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

Published on: April 10, 2009

8.3K

Related Experiment Videos

Last Updated: Dec 31, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses
12:20

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

21.9K
Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

12.5K
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2
10:55

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2

Published on: April 10, 2009

8.3K

Area of Science:

  • Molecular Biology
  • Virology
  • Biotechnology

Background:

  • Baculovirus infection processes are poorly understood due to difficulties in tracking individual viral genomes.
  • Existing methods lack the ability to visualize the entire infection course at the genomic level.

Purpose of the Study:

  • To develop a method for visualizing and tracking individual baculovirus genomes during infection.
  • To adapt ANCHOR™ technology for labeling the Autographa californica Multiple Nucleopolyhedrovirus (AcMNPV) genome.
  • To enable real-time monitoring of baculovirus infection dynamics in living cells and tissues.

Main Methods:

  • Modification of the AcMNPV genome by inserting ANCHOR™ technology components: a DNA-binding protein fused to a fluorescent reporter and its corresponding DNA recognition sequence.
  • Infection of Spodoptera frugiperda (Sf9) cells and larvae with the modified AcMNPV.
  • Monitoring of viral particles, budded viruses, and occlusion bodies using fluorescence.
  • Correlation of fluorescence levels in culture medium with infectious budded virus counts.

Main Results:

  • Generation of a stable, infectious, and replication-competent autofluorescent AcMNPV.
  • Successful visualization and tracking of individual viral genomes and infection progression in living Sf9 cells and larvae.
  • Distinguishable observation of both budded viruses and occlusion bodies.
  • Demonstrated correlation between extracellular fluorescence and infectious budded virus concentration.
  • Design of a reusable cassette for labeling other baculoviruses.

Conclusions:

  • Autofluorescent baculoviruses can be generated using ANCHOR™ technology.
  • This technology provides a powerful tool for studying baculovirus infection dynamics in real-time.
  • The developed method opens new avenues for understanding baculovirus-host interactions and for developing antiviral strategies.