Related Experiment Video
Updated: Jul 10, 2026
Dissection and Culture of Mouse Embryonic Kidney
Published on: May 17, 2017
Expression of the tyrosine kinase receptor EphA5 and its ligand ephrin-A5 during mouse spinal cord development
Christopher P Washburn1, Margaret A Cooper, Renping Zhou
1Susan Lehman Cullman Laboratory for Cancer Research, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, NJ 08854-8020, USA.
Objectives:
To study the expression patterns of two Eph family molecules, the receptor EphA5, and the ligand ephrin-A5, during spinal cord development.
Methods:
The receptor expression was analyzed using beta-galactosidase knockin mice, and affinity ligand probe binding. The ligand expression was assessed using two different affinity probes, and knockout mouse tissues as controls.
Results:
EphA5 was expressed in the ventral spinal cord, while ephrin-A5 was located in the dorsolateral regions of the spinal cord throughout development.
Conclusions:
These results show that EphA5 and ephrin-A5 are expressed over broad developmental stages and may play important roles in establishing the dorsoventral organization of the spinal cord.
More Related Videos
09:53Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
Published on: May 20, 2011
05:36Dissection and Lateral Mounting of Zebrafish Embryos: Analysis of Spinal Cord Development
Published on: February 28, 2014
Related Concept Videos
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
01:51Determination