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Differential interleukin-6 messenger ribonucleic acid expression and its distribution pattern in eutopic and ectopic
Ali Salmassi1, Yahya Acil, Andreas Gerd Schmutzler
1Department of Obstetrics and Gynecology, University Hospitals Schleswig-Holstein, Campus Kiel, Kiel, Germany. endo-office@email.uni-kiel.des
Objective:
To evaluate the concentration and distribution pattern of interleukin-6-producing cells in ectopic and eutopic endometrium.
Design:
Collection of both tissues at the proliferative cycle phase.
Setting:
A university endocrinology and laparoscopy program.
Patient(S):
Twelve samples from six women were obtained at operative laparoscopy in the proliferative phase of each woman's cycle.
Intervention(S):
Paired sampling of ectopic (n = 6) and eutopic endometrium (n = 6).
Main Outcome Measure(S):
Concentration of IL-6 messenger RNA (mRNA) was measured by real-time RT-PCR, and its distribution was determined by in situ RT-PCR.
Result(S):
In vitro RT-PCR analysis showed distinct bands at 234 bp and 115 bp for IL-6 mRNA in eutopic and ectopic endometrium and in placenta as positive control. Real-time RT-PCR indicated significantly higher expression of IL-6 in tissue with endometriosis than in tissue without. In all 12 samples subjected to in situ RT-PCR, positive signals of IL-6 mRNA were observed in cytoplasm of epithelial and stroma cells of ectopic and eutopic endometrium tissue.
Conclusion(S):
The significant IL-6 mRNA increase in endometriosis compared with in eutopic endometrium may play a role in the development of endometriosis and may provide new directions for endometriosis research.
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