Related Experiment Video
Updated: Jul 10, 2026

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
Identification of intact lipid peroxides by Ag+ coordination ion-spray mass spectrometry (CIS-MS)
1Department of Chemistry, Division of Clinical Pharmacology, Center in Molecular Toxicology, Vanderbilt Institute of Chemical Biology, Vanderbilt University, Nashville, Tennessee, USA.
Abstract:
Free radical-induced autoxidation of lipids containing polyunsaturated fatty acids (PUFA) has been implicated in numerous human diseases including atherosclerosis, neurodegenerative diseases, and cancer. Autoxidation of PUFAs generates hydroperoxides as primary oxidation products and further oxidation leads to cyclic peroxides as secondary oxidation products. It is challenging to identify these peroxides by conventional electro-spray ionization (ESI) mass spectrometry (MS) method because of their thermal and chemical instability under most analytical conditions. Ag(+) coordination ion-spray CIS-MS has proven to be a powerful tool to analyze these intact lipid peroxides. Ag(+) preferentially complexes with the double bonds and induces characteristic fragmentation in the gas phase. Monocyclic peroxides, bicyclic endoperoxides, serial cyclic peroxides, and dioxolane-isoprostane peroxides have been identified from the oxidation of cholesteryl arachidonate and phospholipids containing arachidonate. This technique has been widely used for structural identification but it is difficult to make it a quantitative tool because of the formation of multiple silver adducts.
More Related Videos
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)
Published on: May 20, 2013
05:48Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
Published on: September 5, 2014
Related Concept Videos
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Chemical Ionization (CI) Mass Spectrometry
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences
Tandem Mass Spectrometry
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...