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Related Experiment Video

Updated: Jul 10, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

Real-time cell assays of phospholipase A(2)s using fluorogenic phospholipids.

Debasis Manna1, Wonhwa Cho

  • 1Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois, USA.

Methods in Enzymology
|October 24, 2007
PubMed
Summary

New fluorescence assays track Phospholipase A(2) (PLA(2)) activity in real-time. These advanced tools help researchers understand PLA(2) functions and develop targeted inhibitors for various isoforms.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Cell Biology

Background:

  • Phospholipase A(2)s (PLA(2)s) are crucial enzymes regulating lipid mediator production.
  • PLA(2)s are involved in synthesizing arachidonic acid, lysophospholipids, platelet activation factor, and eicosanoids.
  • Understanding PLA(2) isoform-specific functions is vital for numerous physiological processes.

Purpose of the Study:

  • To introduce advanced fluorescence-based assays for real-time cellular PLA(2) activity monitoring.
  • To enable simultaneous measurement of PLA(2) localization and activity with isoform selectivity.
  • To facilitate deeper insights into PLA(2) physiological roles and regulatory mechanisms.

Main Methods:

  • Development of novel fluorogenic phospholipid substrates.

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A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

A Fluorescence-based Assay of Phospholipid Scramblase Activity
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A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

Related Experiment Videos

Last Updated: Jul 10, 2026

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2
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A Liposome Membrane Permeability Assay for Investigating the Effects of Phosphatidylinositol Phosphate Groups on Membranotropic Action of Venom PLA2

Published on: September 26, 2025

A Fluorescence-based Assay of Phospholipid Scramblase Activity
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A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

  • Application of advanced genetic techniques for isoform targeting.
  • Utilization of multiphoton multichannel microscopy for high-resolution imaging.
  • Real-time cellular activity assays for continuous monitoring.

    • Main Results:

    • Simultaneous and continuous measurement of PLA(2) localization and activity is achievable.
    • Isoform-selective analysis of PLA(2) activity in living cells is now possible.
    • The assays provide a powerful tool for studying PLA(2) spatiotemporal regulation.

    Conclusions:

    • These advanced assays significantly enhance the study of diverse PLA(2) isoforms.
    • They are expected to accelerate the discovery of physiological roles and regulatory pathways.
    • The technology aids in developing specific PLA(2) inhibitors and analyzing lipidomics data.