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Microbiological assay for ceftazidime injection.

Andréia De Haro Moreno1, Hérida Regina Nunes Salgado

  • 1Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas, UNESP, Rodovia Araraquara-Jaú, km 1, CEP 14801-302, Araraquara-SP, Brazil.

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A novel biodiffusion assay accurately quantifies ceftazidime in pharmaceutical injections. This microbiological assay provides a reliable method for determining the antibacterial activity of ceftazidime.

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Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Pharmaceutical Science

Background:

  • Accurate quantification of antibiotics is crucial for pharmaceutical quality control.
  • Ceftazidime is a vital third-generation cephalosporin antibiotic.
  • Existing methods for antibiotic quantification may require optimization for specific applications.

Purpose of the Study:

  • To develop a simple, sensitive, and specific biodiffusion assay for quantifying ceftazidime.
  • To validate the assay's performance for pharmaceutical analysis.
  • To establish a reliable microbiological method for antibacterial activity assessment.

Main Methods:

  • Utilized a biodiffusion assay with Staphylococcus epidermidis (ATCC 12228) as the test organism.
  • Measured ceftazidime concentrations in powder for injection (100-400 microg/mL).
  • Performed method validation including linearity, precision, and accuracy assessments.

Main Results:

  • The calibration graph for ceftazidime demonstrated excellent linearity (r² = 1).
  • The assay exhibited high precision (RSD = 0.415) and accuracy.
  • Statistical analysis (linear parallel model, regression analysis, ANOVA) confirmed assay reliability.

Conclusions:

  • The developed biodiffusion assay is a satisfactory method for in vitro quantification of ceftazidime.
  • This microbiological assay is suitable for assessing the antibacterial activity of ceftazidime in pharmaceutical products.
  • The assay offers a simple, sensitive, and specific approach for pharmaceutical quality control.