A New and Ecological Method to Quantify Vancomycin in Pharmaceutical Product by Infrared Spectrometry

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis
• Spectroscopy

Background:

• Existing literature lacks quantitative methods for vancomycin evaluation using infrared spectrometry.
• Infrared spectrometry offers a cleaner analytical alternative, minimizing solvent use and waste generation.

Purpose of the Study:

• To develop and validate a novel, ecological, cost-effective, and rapid infrared spectrometry method for vancomycin quantification.
• To establish a sustainable analytical approach for pharmaceutical product evaluation.

Main Methods:

• Utilized infrared spectrometry with potassium bromide (KBr).
• Focused on the spectral band between 1450-1375 cm⁻¹ for quantitative analysis.
• Validated the method for linearity, selectivity, precision, accuracy, and robustness.

Main Results:

• The method demonstrated linearity in the 1.0-2.0 mg/150 mg range with a high correlation coefficient (0.9994).
• Achieved excellent selectivity, precision (repeatability 2.29%, intermediate precision 3.12%), and accuracy (99.37% recovery).
• The method proved robust against variations in pellet strength, compression time, and KBr brand.

Conclusions:

• The developed infrared spectrometry method is an innovative and clean alternative for quantitative vancomycin analysis.
• The method is fast, user-friendly, economical, non-toxic, and generates minimal waste.
• This validated technique is suitable for routine analysis of vancomycin dosage forms, promoting sustainable pharmaceutical analysis.