Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Oxidative Cleavage of Alkenes: Ozonolysis01:46

Oxidative Cleavage of Alkenes: Ozonolysis

In ozonolysis, ozone is used to cleave a carbon–carbon double bond to form aldehydes and ketones, or carboxylic acids, depending on the work-up.
Ozone is a symmetrical bent molecule stabilized by a resonance structure.
Atomic Absorption Spectroscopy: Atomization Methods01:25

Atomic Absorption Spectroscopy: Atomization Methods

Atomic Absorption Spectroscopy (AAS) atomizes samples through flame atomization or electrothermal atomization. Flame atomization typically involves a nebulizer and spray chamber assembly to combine the sample with a fuel–oxidant mixture, creating a fine aerosol mist that enters a burner. Typically, the fuel and oxidant are combined in an approximately stoichiometric ratio. However, for atoms that are easily oxidized, a fuel-rich mixture may be more advantageous. Only about 5% of the aerosol...
Spectroscopy of Carboxylic Acid Derivatives01:26

Spectroscopy of Carboxylic Acid Derivatives

Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and unsymmetrical carbonyl vibration.
In the...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrating computerized clinical decision support systems into clinical work: A meta-synthesis of qualitative research.

International journal of medical informatics·2015
Same author

Comment on "Retrieval practice produces more learning than elaborative studying with concept mapping".

Science (New York, N.Y.)·2011
Same author

Initial investigation of acoustic droplet vaporization for occlusion in canine kidney.

Ultrasound in medicine & biology·2010
Same author

Quantitative analysis using Raman spectrometry.

Applied spectroscopy·2003
Same author

Raman sensitivity enhancement for aqueous protein samples using a liquid-core optical-fiber cell.

Analytical chemistry·2001
Same author

Time correlation method for measuring fluorescence decays with a cw laser.

The Review of scientific instruments·1979

Related Experiment Video

Updated: Jul 10, 2026

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example
09:56

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Published on: November 18, 2015

On-line analysis of a continuous-flow ozonolysis reaction using Raman spectroscopy.

M J Pelletier1, M L Fabiilli, Brian Moon

  • 1Research Analytical, Pharmaceutical Sciences, Pfizer Global Research and Development, 2800 Plymouth Road, Ann Arbor, Michigan 48105, USA. Michael.Pelletier@pfizer.com

Applied Spectroscopy
|October 26, 2007
PubMed
Summary

This study introduces an on-line Raman analyzer for real-time monitoring of ozonolysis reactions. It accurately quantifies key compounds, outperforming traditional off-line methods by avoiding sampling bias.

More Related Videos

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

Analyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry
08:23

Analyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry

Published on: June 14, 2018

Related Experiment Videos

Last Updated: Jul 10, 2026

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example
09:56

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Published on: November 18, 2015

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer
07:52

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

Published on: April 12, 2017

Analyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry
08:23

Analyzing the Photo-oxidation of 2-propanol at Indoor Air Level Concentrations Using Field Asymmetric Ion Mobility Spectrometry

Published on: June 14, 2018

Area of Science:

  • Analytical Chemistry
  • Chemical Engineering
  • Spectroscopy

Background:

  • Ozonolysis reactions are crucial in atmospheric chemistry and organic synthesis.
  • Accurate real-time monitoring of reaction intermediates and products is essential for process optimization.
  • Traditional off-line analysis methods can introduce significant bias due to sampling procedures.

Purpose of the Study:

  • To develop and validate an on-line Raman spectroscopy method for quantitative analysis of ozonolysis reactions.
  • To compare the performance of spectral stripping with partial least squares (PLS) analysis for quantitative tracking.
  • To investigate the impact of process variables like feed flow rate and ozone concentration on the reaction.

Main Methods:

  • Utilized an on-line Raman analyzer integrated with a continuous flow ozonolysis reactor.
  • Employed spectral stripping for quantitative analysis, incorporating prior knowledge to address baseline artifacts.
  • Compared spectral stripping performance against partial least squares (PLS) regression.
  • Systematically varied feed flow rate and ozone concentration to study their effects.

Main Results:

  • Successfully demonstrated quantitative tracking of trans-stilbene, benzaldehyde, and alpha-methoxybenzyl hydroperoxide.
  • Spectral stripping provided accurate quantification, effectively overcoming baseline interferences.
  • The study highlighted the bias introduced by off-line sampling compared to the on-line approach.
  • Process variables were shown to influence reaction dynamics.

Conclusions:

  • On-line Raman analysis with spectral stripping is a robust and accurate method for monitoring continuous flow ozonolysis.
  • This technique offers significant advantages over traditional off-line methods by providing real-time data and minimizing sampling bias.
  • The findings support the adoption of on-line analytical tools for enhanced process control and understanding in chemical reactions.