Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vacuum-enhanced high-resolution 3D printing yields 11 200 valves and uniform 7 μm isoporous membranes.

Lab on a chip·2026
Same author

Chemically Selective Nanoelectrode Arrays for Real-Time, Parallel Neurotransmitter and Electrical Recording.

Small science·2026
Same author

Fast multi-resolution 3D printing of microfluidics: enabling 2 μm channels and ultra-compact mixers.

Microsystems & nanoengineering·2026
Same author

Advancing the Applications of 3D Printed Microfluidics: Utilizing Quantum Dots to Measure Internal Temperature.

International journal of heat and mass transfer·2025
Same author

From biology to circuitry: a review of DNA and other biomaterials as templates for nanoelectronic systems.

Chemical communications (Cambridge, England)·2025
Same author

Extruded filament electrodes for lactate biosensing in continuous-injection paper-based microfluidic devices.

Biosensors & bioelectronics·2025

Related Experiment Video

Updated: Jul 10, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Electrically actuated, pressure-driven liquid chromatography separations in microfabricated devices.

Hernan V Fuentes1, Adam T Woolley

  • 1Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.

Lab on a Chip
|October 26, 2007
PubMed
Summary

Electrolysis-based micropumps in microfluidic devices enable rapid, picoliter-scale liquid chromatography separations. These integrated systems offer a significant advancement for miniaturized, fully automated analytical platforms.

More Related Videos

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification
07:11

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Published on: February 27, 2026

Related Experiment Videos

Last Updated: Jul 10, 2026

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
11:32

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification
07:11

Automated HPLC Separation Using LC-Mate: An Integrated Repetitive Autosampler and Fraction Collector for Microscale Purification

Published on: February 27, 2026

Area of Science:

  • Microfluidics
  • Analytical Chemistry
  • Materials Science

Background:

  • Microfluidic devices are increasingly utilized for miniaturized analytical separations.
  • Electrochemical pumping offers a potential alternative to traditional fluidic control methods in microdevices.

Purpose of the Study:

  • To develop and demonstrate electrolysis-based micropumps integrated with microfluidic channels for rapid chromatographic separations.
  • To investigate the performance and limitations of these integrated systems for picoliter sample loading and analysis.

Main Methods:

  • Fabrication of multi-layer microfluidic devices using photolithography, wet etching, and thermal bonding.
  • Integration of electrolysis-based micropumps for precise fluid control and sample injection.
  • Development of a stationary phase coating within microchannels for chromatographic separation.
  • Optimization of electrochemical parameters for pressure-balanced injection and bubble management.

Main Results:

  • Successful fabrication of integrated microfluidic devices with on-chip electrochemical micropumps.
  • Demonstration of picoliter sample loading with no dead volume.
  • Rapid chromatographic separation of three fluorescently labeled amino acids in under 40 seconds.
  • Achieved high separation efficiency (>3000 theoretical plates) in a short channel (2.5 cm).
  • Identified current fluctuations due to bubble formation as a key source of separation variability.

Conclusions:

  • Electrochemical micropumps integrated with microfluidic channels show significant potential for rapid chromatographic separations.
  • These devices represent a crucial step towards developing miniaturized and fully integrated liquid chromatography systems.
  • Further optimization is needed to mitigate bubble formation and enhance separation reproducibility.