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Related Experiment Videos

Multiple internal reflection poly(dimethylsiloxane) systems for optical sensing.

A Llobera1, S Demming, R Wilke

  • 1Institut für Mikrotechnik, Technische Universität Braunschweig, Alte Salzdahlumer Strasse 203, 38124 Braunschweig, Germany. andreu.llobera@cnm.es

Lab on a Chip
|October 26, 2007
PubMed
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New poly(dimethylsiloxane)-based (PDMS) systems enhance absorbance measurements using multiple internal reflections. These systems achieve high sensitivity and low detection limits for chemical analysis.

Area of Science:

  • Analytical Chemistry
  • Optical Engineering
  • Materials Science

Background:

  • Highly sensitive absorbance measurements are crucial for chemical analysis.
  • Traditional flow cell designs often face limitations in sensitivity and volume.
  • Poly(dimethylsiloxane) (PDMS) offers a versatile platform for microfluidic device fabrication.

Purpose of the Study:

  • To develop compact PDMS-based multiple internal reflection systems for enhanced absorbance measurements.
  • To optimize optical path length within microfluidic flow cells without significantly increasing volume.
  • To investigate the impact of air mirrors on sensitivity and detection limits.

Main Methods:

  • Design and simulation of propagating multiple internal reflection (PMIR) systems using ray-tracing.

Related Experiment Videos

  • Fabrication of PDMS microfluidic devices incorporating self-alignment, lenses, mirrors, and microfluidic channels.
  • Experimental characterization of PMIR systems with fluorescein in phosphate buffer.
  • Development and testing of a ring-shaped multiple internal reflection (RMIR) configuration.

    • Main Results:

    • Experimental results validated ray-tracing predictions for PMIR systems.
    • Sensitivity was increased by lengthening the optical path using strategically placed air mirrors.
    • A limit of detection (LOD) of 110 nM was achieved for fluorescein.
    • The RMIR configuration, with an additional air mirror, reduced the LOD to 41 nM and decreased sensing region length.

    Conclusions:

    • PDMS-based PMIR systems offer a viable approach for highly sensitive absorbance measurements.
    • Optimizing the sensing region and incorporating air mirrors significantly enhances detection capabilities.
    • The RMIR configuration provides a more compact and sensitive solution for trace analysis.