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Immunolocalization of farnesoid X receptor (FXR) in mouse tissues using tissue microarray
Hiroyuki Higashiyama1, Mine Kinoshita, Satoshi Asano
1Tsukuba Research Laboratories, Pharmacology Department, GlaxoSmithKline, 43 Wadai, Tsukuba, Ibaraki 300-4247, Japan.
Acta Histochemica
|October 30, 2007
Summary
Farnesoid X receptor (FXR) is a nuclear receptor involved in metabolism. This study mapped FXR and RXR-alpha localization in mice, revealing FXR
Area of Science:
- Nuclear receptor signaling
- Molecular biology
- Physiology
Background:
- Farnesoid X receptor (FXR) is a nuclear receptor crucial for bile acid, lipid, and glucose metabolism.
- Understanding FXR's systemic physiological roles requires detailed localization studies.
Purpose of the Study:
- To elucidate the systemic physiological functions of FXR by mapping its cellular and subcellular localization in adult mice.
- To investigate the localization of FXR's heterodimer partner, retinoid X receptor (RXR)-alpha.
Main Methods:
- Comprehensive immunohistochemical analysis of FXR and RXR-alpha localization.
- Utilized tissue microarray (TMA)-based immunohistochemistry in adult mice tissues.
Main Results:
- FXR immunolabeling was detected in the enterohepatic system (intestines, hepatocytes, gall bladder).
- FXR was also found in epithelial cells of the tongue, esophagus, forestomach, skin, eye (cornea, ciliary body), and adrenocortical cells.
- FXR predominantly localized to the nucleus in positive cells, while RXR-alpha was ubiquitously nuclear.
Conclusions:
- FXR exhibits diverse physiological roles beyond metabolism, potentially impacting epithelial barriers, vision, and urinary function.
- FXR's nuclear localization suggests direct transcriptional regulation in various tissues.
- Co-localization with RXR-alpha supports their functional partnership across multiple organ systems.
