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[Interspecies mouse-mink hybridomas as producers of mink immunoglobulin]
The work is aimed at establishing the interspecies mouse-mink hybridomas from the fusion of American mink B-lymphocytes with the murine cell line NSO. The hybridoma (lime 10-B5) continued to secrete mink immunoglobulin L-chains in the culture for 6 months with constant reclonings. The hybridoma clone was characterized by a decrease in the secretory activity of cells. The karyological study et this clone has not reliably revealed the mink chromosomes in the genome of hybrid cells.
The work is aimed at establishing the interspecies mouse-mink hybridomas from the fusion of American mink B-lymphocytes with the murine cell line NSO. The hybridoma (lime 10-B5) continued to secrete mink immunoglobulin L-chains in the culture for 6 months with constant reclonings. The hybridoma clone was characterized by a decrease in the secretory activity of cells. The karyological study et this clone has not reliably revealed the mink chromosomes in the genome of hybrid cells.