Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bioinformatics approaches to quadruplex sequence location.

Alan K Todd1

  • 1CRUK Biomolecular Structure Group, University of London, School of Pharmacy, 29-39 Brunswick Square, London WC1N 1AX, USA. alan.todd@pharmacy.ac.uk

Methods (San Diego, Calif.)
|October 31, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A naphthalene diimide G-quadruplex ligand inhibits cell growth and down-regulates BCL-2 expression in an imatinib-resistant gastrointestinal cancer cell line.

Bioorganic & medicinal chemistry·2018
Same author

Cluster stability in the analysis of mass cytometry data.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2016
Same author

Crystal structure of a promoter sequence in the B-raf gene reveals an intertwined dimer quadruplex.

Journal of the American Chemical Society·2013
Same author

Small-molecule G-quadruplex interactions: Systematic exploration of conformational space using multiple molecular dynamics.

Biopolymers·2013
Same author

Sequences in the HSP90 promoter form G-quadruplex structures with selectivity for disubstituted phenyl bis-oxazole derivatives.

Bioorganic & medicinal chemistry letters·2012
Same author

A novel series of G-quadruplex ligands with selectivity for HIF-expressing osteosarcoma and renal cancer cell lines.

Bioorganic & medicinal chemistry letters·2012
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Identifying genomic sequences that form guanine quadruplex structures is crucial for therapeutic development. Current strategies analyze sequence and genomic location, but further experimentation is needed to confirm physiological relevance.

Area of Science:

  • Genomics
  • Structural Biology
  • Bioinformatics

Background:

  • Guanine quadruplex (G4) structures are increasingly recognized as significant therapeutic targets.
  • Numerous genomic sequences with the potential to form G4 structures have been identified.
  • Distinguishing physiologically relevant G4 structures from mere sequence potential is a key challenge.

Purpose of the Study:

  • To review strategies for identifying and prioritizing physiologically relevant guanine quadruplex forming sequences.
  • To highlight the limitations of sequence-based identification alone.
  • To emphasize the need for integrated approaches combining sequence analysis with structural and genomic context.

Main Methods:

  • Comparative analysis of G4 forming sequences within different genomic regions.

Related Experiment Videos

  • Utilizing limited available structural information of G4s.
  • Bioinformatic approaches to predict G4 formation potential.

    • Main Results:

    • A high number of potential G4 forming sequences exist in the genome.
    • Sequence analysis alone is insufficient for determining G4 structure formation in vivo.
    • Genomic location and comparative analyses provide initial filtering for relevant sequences.

    Conclusions:

    • Guanine quadruplex structures hold therapeutic promise.
    • Current methods for identifying relevant G4 sequences require refinement.
    • Combining sequence data with genomic context and experimental validation is essential for discovering functional G4 structures.