Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

DNA damaging properties of G-quadruplex ligand QN-302 are potentiated by the DNA repair inhibitor Olaparib and mitigated by the molecular helicase PhpC.

Genome biology·2026

Same author

Environmental Dipolar Relaxation during Excited-State Proton Transfer in Green Fluorescent Protein.

Journal of the American Chemical Society·2026

Same author

Structure, Function and Dynamics of mCoral, a pH-Responsive Engineered Variant of the mCherry Fluorescent Protein with Improved Hydrogen Peroxide Tolerance.

International journal of molecular sciences·2026

Same author

Energy Upconversion Using Platinum(II)-BPI Photosensitizers.

Inorganic chemistry·2025

Same author

Stereogenic-at-Metal Ir(III) Complexes as Platforms for the Construction of Asymmetric Bimetallic Complexes.

Inorganic chemistry·2025

Same author

The Baeyer-Villiger Oxidation of Cubyl Ketones: A Synthetic Route to Functionalized Cubanols.

Organic letters·2025

Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026

Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026

Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026

Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026

Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026

Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 10, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Small-molecule G-quadruplex interactions: Systematic exploration of conformational space using multiple molecular

Jarmila Husby¹, Alan K Todd, James A Platts

¹School of Pharmacy, University College London, London, WC1N 1AX, UK.

|July 6, 2013

Summary

This summary is machine-generated.

G-quadruplexes are flexible DNA structures. A new computational method combining molecular dynamics and sampling addresses this flexibility, aiding the discovery of new quadruplex-binding molecules.

Keywords:

dna drug quadruplex

More Related Videos

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Related Experiment Videos

Last Updated: May 10, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

In Vitro Chemical Mapping of G-Quadruplex DNA Structures by Bis-3-Chloropiperidines

Published on: May 12, 2023

Area of Science:

Genomics
Structural Biology
Computational Chemistry

Background:

G-quadruplexes are four-stranded nucleic acid structures crucial in various genomic regions.
Their conformational flexibility, particularly in loop regions, hinders structure-based drug design.
Targeting G-quadruplexes with small molecules is a significant area of therapeutic interest.

Purpose of the Study:

To present a novel computational approach to overcome the challenge of G-quadruplex conformational flexibility.
To facilitate the discovery and optimization of small molecules that bind to G-quadruplexes.

Main Methods:

A combined multiple molecular dynamics and sampling approach was employed.
The method was tested using two small molecules, RHPS4 and pyridostatin, with varying flexibility.

Main Results:

The developed computational strategy effectively addresses the conformational flexibility of G-quadruplexes.
This approach provides a robust framework for structure-based design of G-quadruplex ligands.

Conclusions:

The presented method offers a solution for studying flexible G-quadruplex structures.
This advancement is expected to accelerate the development of novel therapeutics targeting G-quadruplexes.