Construction of simplified boundary surfaces from serial-sectioned metal micrographs
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A Method for Obtaining Serial Ultrathin Sections of Microorganisms in Transmission Electron Microscopy
Published on: January 17, 2018
Scott Dillard1, John Bingert, Dan Thoma
1Institute for Data Analysis and Visualization, University of California, Davis, USA. sedillard@ucdavis.edu
Abstract:
We present a method for extracting boundary surfaces from segmented cross-section image data. We use a constrained Potts model to interpolate an arbitrary number of region boundaries between segmented images. This produces a segmented volume from which we extract a triangulated boundary surface using well-known marching tetrahedra methods. This surface contains staircase-like artifacts and an abundance of unnecessary triangles. We describe an approach that addresses these problems with a voxel-accurate simplification algorithm that reduces surface complexity by an order of magnitude. Our boundary interpolation and simplification methods are novel contributions to the study of surface extraction from segmented cross-sections. We have applied our method to construct polycrystal grain boundary surfaces from micrographs of a sample of the metal tantalum.

