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Related Experiment Video

Updated: Jul 10, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Lactoyl-poloxamine/collagen matrix for cell-containing tissue engineering modules.

Alejandro Sosnik1, Brendan M Leung, Michael V Sefton

  • 1Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, Terrence Donelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada M5S 3E1.

Journal of Biomedical Materials Research. Part A
|October 31, 2007
PubMed
Summary

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New biodegradable poloxamine scaffolds show promise for tissue engineering. These lactoyl-poloxamine derivatives support cell growth and show potential for creating remodelable tissue scaffolds.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Developing advanced biomaterials is crucial for tissue engineering.
  • Poloxamines are versatile polymers with potential for modification.
  • Biodegradable scaffolds are needed for applications requiring tissue remodeling.

Purpose of the Study:

  • To synthesize and characterize novel collagen-containing, remodelable poloxamine derivatives.
  • To evaluate the degradation properties of these new materials.
  • To assess the cytocompatibility and cellular response within these poloxamine-based scaffolds for tissue engineering.

Main Methods:

  • Poloxamine modification with oligo(lactic acid) segments and methacryloyl chloride.
  • In vitro degradation studies using disc samples.

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Last Updated: Jul 10, 2026

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment
07:12

Microengineering 3D Collagen Hydrogels with Long-Range Fiber Alignment

Published on: September 7, 2022

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration
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Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

  • Cell encapsulation (HepG2, UVSMC) and viability assessment (Live/Dead assay).
  • Morphological analysis of cell-laden scaffolds over time.
  • Evaluation of endothelial cell adhesion and coverage on UVSMC-seeded scaffolds.

    • Main Results:

    • Synthesized poloxamine derivatives demonstrated controlled degradation with faster weight loss compared to controls.
    • High cell viability (HepG2, UVSMC) was observed after crosslinking.
    • Lactoyl-poloxamine matrices exhibited shrinkage and increased surface roughness, consistent with degradation.
    • Embedded UVSMC cells adopted characteristic shapes, and primary UVSMC formed clusters.
    • Scaffolds with embedded UVSMC enhanced initial endothelial cell adhesion and surface coverage.

    Conclusions:

    • Lactoyl-poloxamine derivatives are biodegradable and cytocompatible, suitable for cell encapsulation.
    • The observed degradation and cellular responses indicate potential for remodelable tissue engineering scaffolds.
    • Further optimization may lead to advanced applications in modular tissue engineering where tissue remodeling is key.