[Simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus by high...

A high performance liquid chromatographic (HPLC) method with gradient elution was developed for the simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus. The analytes were separated on a Waters (5)C18-MS-II column (4.6 mm x 250 mm) and detected at 220 nm. The mobile phases were methanol-1% (v/v) diethylamine solution (adjusted to pH 7.3 with phosphate) for gradient elution at temperature of 25 degrees C. The calibration curves for vindoline, catharanthine and anhydrovinblastine showed good linearity in the ranges of 0.03-1 mg/mL (r = 0.9997), 0.03-1 mg/mL (r = 0.9999) and 0.01-0.5 mg/mL (r = 0.9986), respectively. The recoveries were 96.8%, 97.0%, and 96.4% and the relative standard deviations (RSDs) were 1.53%, 1.37%, and 1.96%. The method is accurate, rapid and simple for the determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus.