Related Experiment Video
Updated: Jul 10, 2026

The Use of Gas Chromatography to Analyze Compositional Changes of Fatty Acids in Rat Liver Tissue during Pregnancy
Published on: March 13, 2014
Separation of short-chain fatty acids on a gas chromatographic column coated with oxidized lubricating oil
1Egyptian Petroleum Research Institute, Nasr City, Cairo, Egypt. nemoustaf@yahoo.com
Abstract:
The oxidized lubricating naphthenic base oil was used as a stationary phase for the separation of short-chain free fatty acids (SFFA) either as a pure sample or an aqueous solution containing 0.9-1.2 mg/L of each acid. It is found that the oil oxidation for 20 h improved the separation of SFFA in these two sample forms. This separation improvement represents not only the increase in retention volume intervals and peak symmetries in case of the pure sample but also in acid peak areas in case of the aqueous solution sample.
More Related Videos
13:02Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)
Published on: March 18, 2011
11:44Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
Published on: March 6, 2016
Related Concept Videos
Supercritical Fluid Chromatography
SFC utilizes a supercritical fluid mobile phase,...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Gas Chromatography: Introduction
In GC, a sample is vaporized and mixed with an inert carrier gas (the mobile phase), which transports it through a column.
Thin-Layer Chromatography (TLC): Overview
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall. The coating...