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Updated: Jul 10, 2026
Examining Proteasome Assembly with Recombinant Archaeal Proteasomes and Nondenaturing PAGE: The Case for a Combined Approach
Published on: December 17, 2016
[Preliminary analyses for influence of mutant PrPs with different number of octapeptide]
Lu Han1, Yan-Zhen Wan, Jun Han
1Department of Prion Disease, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Objective:
The present study was conducted to understand the effects of PrP in different octapeptide repeats on proliferation of HeLa cells.
Methods And Results:
Mutant PrPs with octapeptide repeat insertion were transiently expressed in HeLa cells and their results of MTT assay showed stronger cytotoxic effect on the proliferation of cells than wild-type PrP. Annexin V/PI assay also demonstrated that the expression of mutant PrPs was much easier to induce apoptosis than wild-type in HeLa cells. The percentage of both early and late stage apoptosis in mutant groups were significantly higher than that of wild type.
Conclusion:
These data suggest that the expression of mutant PrPs associated with familial CJD is much easier to induce apoptosis in cultured cells than expression of wild type PrP.
