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Identification of protease substrates by combinatorial profiling on TentaGel beads
Jacob Kofoed1, Jean-Louis Reymond
1Department of Chemistry and Biochemistry, University of Berne, Freiestrasse 3, CH-3012 Berne, Switzerland.
Chemical Communications (Cambridge, England)
|November 1, 2007
Abstract:
Reacting a 65,536 member combinatorial library of octapeptides on TentaGel beads with various proteases followed by selective staining of the free amino termini at the reacted bead surface and sequence determination by amino acid analysis allowed the rapid identification of protease substrates.
