Related Experiment Video
Updated: Jul 10, 2026

Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles
Published on: March 9, 2019
Isomeric control of protein recognition with amino acid- and dipeptide-functionalized gold nanoparticles
Chang-Cheng You1, Sarit S Agasti, Vincent M Rotello
1Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, MA 01002, USA.
Abstract:
Amino acid and dipeptide-functionalized gold nanoparticles (NPs) possessing L/D-leucine and/or L/D-phenylalanine residues have been constructed in order to target the surfaces of alpha-chymotrypsin (ChT) and cytochrome c (CytC). Isothermal titration calorimetry (ITC) was conducted to evaluate the binding thermodynamics and selectivity of these NP-protein interactions. The chirality of the NP end-groups substantially affects the resultant complex stability, with up to 20-fold differences seen between particles of identical hydrophobicity, demonstrating that structural information from the ligands can be used to control protein recognition.
Related Concept Videos
Immunogold Electron Microscopy
Protein-protein Interfaces

