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[Study on RP-HPLC fingerprint of xinshao injection]
Hong Su1, Ai-min Wang, Yong-lin Wang
1School of Pharmacy, Guiyang Medical College, Guiyang 550004, China.
Objective:
To establish the fingerprint detecting standard of xinshao Injection by reversed phase high performance liquid chromatography.
Method:
The chromatographic conditions were as follows: an Diamonsil C18 column was used, the mobile phase was composed of 80% acetontrile and 0.1% phosphoric acid with gradient elution, the flow rate 1.0 mL min(-1), column temperature was 40 degrees and the UV absorbance detection was set at 230 nm.
Result:
A standard HPLC fingerprint procedure was developed for xinshao Injection, with 8 common peaks. Under the same selected chromatographic conditions, the HPLC fingerprints of raw drugs and their intermediate, finished products were obtained with good separation and correlation (no less than 0.97) according to the technical requirements of fingerprint on Injection of Chinese traditional medicine.
Conclusion:
This method was accurate, repeatable and can be used as the important parameters of the quality control for xinshao injection.
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