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[Processing method and HPLC fingerprint of charred radix Scutellariae]
Yun Yang1, Yong-ju Wen, Jun-min Wang
1Henan College of Traditional Chinese Medicine, Henan, Zhengzhou 450008, China. yyun@china.com.cn
Objective:
To investigate the process and HPLC fingerprint of Charred Radix Scutellariae, and lay a foundation of Charred Radix Scutellariae quanlity control mode.
Method:
Select HPLC-UV fingerprint. Chromatogram condition: Hypersil C18 column (5.0 mm x 200 mm, 5 microm), mixtures of methanol, 0.4% phosphoric acid and acetonitrile as mobile phase in a gradient mode. Flow rate: 1.0 mL x min; Detection wavelength was set at 277 nm.
Result:
There were no evident differences among Charred Radix Scutellariae that were normatively manufactured and processed.
Conclusion:
This test proves the process is feasible, this method recurs well and can be used to provide scieitific basis for the normative process and quanlity control mode of Charred Radix Scutellariae.
