Related Experiment Video
Updated: Jul 10, 2026
Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds
Published on: January 7, 2019
Development of 3-D nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone
Chang Seok Ki1, Sook Young Park, Hyun Jeong Kim
1Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, Republic of Korea.
Abstract:
We made a three-dimensional (3-D) nanofibrous fibroin scaffold (NFS) with high porosity (94%) and examined its feasibility in bone regeneration. Under scanning electron microscopy, MC3T3-E1 preosteoblasts on the scaffold showed more spread on the first day after seeding compared with a 2-D scaffold. MTT assay showed significantly increased proliferation in 3-D NFS compared with 2-D NFS 7 days after seeding (P < 0.05). Western immunoblotting for activated paxillin, FAK, AKT, C-Src, and ERK1/2 antibodies showed signals from the extracellular matrix were significantly increased in 3-D NFS. Newly developed 3-D electrospun NFS may be a good candidate for use in bone regeneration.
