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Updated: Jul 10, 2026
Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
Published on: February 1, 2019
PEGylated lipoplexes: preparation protocols affecting DNA condensation and cell transfection efficiency
Cecilia Bombelli1, Francesca Faggioli, Paola Luciani
1Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy.
Abstract:
The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a psi phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.
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