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Published on: May 30, 2015
A more efficient enzyme-linked immunosorbent assay for measurement of alpha-synuclein in cerebrospinal fluid
Wieneke J A van Geel1, W Farid Abdo, René Melis
1Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Centre, The Netherlands.
Abstract:
We describe a modification of a previously described assay for the quantification of alpha-synuclein in naive cerebrospinal fluid, which allows for a more efficient quantification of alpha-synuclein. Detection limit of the assay is 3.8 ng/ml and the assay is linear until 300 ng/ml. Inter-assay and intra-assay coefficients of variation are below 15% in a wide range of concentrations. Mean recovery of the assay is 94%. The 95% upper limit of the reference range (p95) in a group of neurological controls above the age of 45 years is 62 ng/ml. This assay can be routinely applied for quantification of alpha-synuclein in cerebrospinal fluid, but not in serum, and this may serve as a possible biomarker for alpha-synucleinopathies such as Parkinson's disease and multiple system atrophy.
