Related Experiment Video
Updated: Jul 10, 2026

Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
Published on: March 17, 2023
Microbiologic evaluation of microfiber mops for surface disinfection.
William A Rutala1, Maria F Gergen, David J Weber
1Department of Hospital Epidemiology, University of North Carolina School of Medicine, Chapel Hill, NC 27599-7030, USA. brutala@unch.unc.edu
Microfiber mops significantly reduce floor microbes compared to cotton mops. Disinfectant use offers no added benefit with microfiber systems, but improves cotton mop efficacy.
Area of Science:
- Healthcare-associated infection control
- Environmental hygiene in healthcare settings
Background:
- Healthcare facilities are increasingly adopting microfiber mopping systems.
- This shift aims to improve floor sanitation over traditional cotton string mops.
Purpose of the Study:
- To evaluate the microbial reduction efficacy of microfiber mops.
- To compare microfiber mop performance against conventional cotton string mops.
Main Methods:
- Three test conditions were evaluated: cotton mop with bucket, microfiber mop with bucket, and a microfiber system.
- Rodac plates with D/E Neutralizing Agar assessed microbial levels before and after cleaning in 24 rooms per condition.
Main Results:
- The microfiber system achieved 95% microbial removal with detergent, significantly outperforming cotton mops (68%).
- Adding disinfectant to the microfiber system did not increase microbial removal (95% vs. 95%).
- Disinfectant use significantly improved cotton mop efficacy (95% vs. 68%).
Conclusions:
- Microfiber systems provide superior microbial reduction on floors compared to cotton mops when using detergent.
- Disinfectant addition does not enhance the microbial elimination capabilities of microfiber mopping systems.
Related Concept Videos
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Antimicrobial Effectiveness
Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
Hand hygiene
Hand washing...
Chemical Agents for Microbial Control

