Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin homology) domains...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pediatric Digital Artery Pseudoaneurysm From Repetitive Compression While Gripping Toys.

Plastic and reconstructive surgery. Global open·2026
Same author

Utility of breath-hold half-Fourier single-shot turbo spin-echo with deep learning-based reconstruction for acquiring fat-suppressed T2-weighted images of the pancreas.

European journal of radiology open·2026
Same author

Eliminating Post-Operative Pain with Intercostal Nerve Cryoablation during Rib Cartilage Harvest for Total Microtia Construction.

Plastic and reconstructive surgery·2026
Same author

Repeatability, reproducibility, and agreement regarding measurement of choroidal vascularity index between OCT and OCT angiography.

International journal of ophthalmology·2026
Same author

Response to Dr. S Dhanya Dedeepya' s letter.

Japanese journal of radiology·2026
Same author

Characterization and Management of a Rare Recurrent Pediatric Papillary Hemangioma.

Plastic and reconstructive surgery. Global open·2026

Related Experiment Video

Updated: Jul 10, 2026

Stretching Micropatterned Cells on a PDMS Membrane
09:41

Stretching Micropatterned Cells on a PDMS Membrane

Published on: January 22, 2014

Analysis of cyclic-stretching responses using cell-adhesion-patterned cells.

Yuki Katanosaka1, Jin-Hua Bao, Tomoyo Komatsu

  • 1Department of Cardiovascular Physiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.

Journal of Biotechnology
|November 6, 2007
PubMed
Summary

Researchers developed a novel method using patterned fibronectin dots to study how mechanical forces affect human vascular endothelial cells (HUVECs). This technique allows for precise analysis of cellular responses like tyrosine phosphorylation and actin polymerization under cyclic strain.

More Related Videos

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
25:12

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Related Experiment Videos

Last Updated: Jul 10, 2026

Stretching Micropatterned Cells on a PDMS Membrane
09:41

Stretching Micropatterned Cells on a PDMS Membrane

Published on: January 22, 2014

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain
25:12

Mechanical Stimulation of Stem Cells Using Cyclic Uniaxial Strain

Published on: July 29, 2007

Area of Science:

  • Cell Biology
  • Biomaterials Science
  • Mechanobiology

Background:

  • Human vascular endothelial cells (HUVECs) are crucial for blood vessel function and respond to mechanical forces.
  • Previous studies showed cyclic strain induces tyrosine phosphorylation and mitogen-activated protein kinase (MAPK) activation in HUVECs.
  • Quantifying spatial signaling in randomly adhering cells is challenging.

Purpose of the Study:

  • To develop a system for analyzing the spatial distribution of tyrosine phosphorylation and actin polymerization in HUVECs under mechanical strain.
  • To overcome limitations in quantifying cellular responses in randomly adhering cells.

Main Methods:

  • Utilized microcontact printing to create uniform, 2 µm fibronectin dot patterns on polydimethylsiloxane (PDMS) stretch chambers.
  • Seeded HUVECs onto these patterned substrates, allowing controlled attachment and morphology.
  • Applied cyclic, uni-axial strain to the patterned cells and analyzed tyrosine phosphorylation and actin polymerization using fluorescent microscopy.

Main Results:

  • HUVECs successfully attached to the patterned fibronectin dots, exhibiting uniform size and morphology.
  • The developed system enabled statistically sound analysis of cellular responses to cyclic strain.
  • Spatial distribution of tyrosine phosphorylation and actin polymerization could be quantified in patterned HUVECs.

Conclusions:

  • The novel patterned cell system provides a robust platform for mechanobiology research.
  • This method facilitates quantitative analysis of signaling pathways in endothelial cells under mechanical stress.
  • Enables deeper understanding of how physical forces regulate endothelial cell behavior and function.