Multiple splicing results in at least two p203 proteins that are expressed in the liver and down-regulated during

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Yanjun Zhang1, Qingyun Tian, Yi Du

  • 1Life Sciences College, Shandong University, Jinan, Shandong 250100, PR China.

Frontiers in Bioscience : a Journal and Virtual Library
|November 6, 2007
PubMed

Insights

The interferon-inducible p203 protein, a member of the p200 family, is exclusively expressed in the liver. Its levels decrease during liver regeneration before cell cycle progression.

Area of Science:

  • Molecular Biology
  • Immunology
  • Cell Biology

Background:

  • Interferon-inducible p200 family proteins regulate cell proliferation and differentiation.
  • The specific function of p203, a p200 family member, remains largely unknown.
  • p203 isoforms and their expression patterns require further investigation.

Purpose of the Study:

  • To characterize the interferon-inducible p203 (Ifi203) gene and its protein products.
  • To investigate the expression pattern of p203 in different cell types and mouse strains.
  • To explore the role of p203 during liver regeneration.

Main Methods:

  • Cloning of four Ifi203 splicing isoforms from interferon-induced 10T1/2 cells.
  • Preparation of anti-p203 antiserum for immunoblot detection of p203 proteins.
  • Analysis of p203 expression in various cell lines and tissues from C129/SvJ and C57BL/6 mice.
  • Monitoring p203 levels during liver regeneration after partial hepatectomy in C57BL/6 mice.

Main Results:

  • Four splicing isoforms of Ifi203 were identified and cloned.
  • Immunodetection revealed p203 proteins of 46 kD and 51 kD in multiple cell lines.
  • p203 exhibited exclusive expression in the liver of adult C129/SvJ and C57BL/6 mice.
  • p203 levels significantly decreased 6-24 hours post-hepatectomy, preceding G1/S phase transition during liver regeneration.

Conclusions:

  • p203 is a liver-specific interferon-inducible protein with distinct expression patterns.
  • The dynamic changes in p203 levels during liver regeneration suggest a role in regulating cell cycle progression.
  • Further research is warranted to elucidate the precise molecular mechanisms of p203 function in liver biology and regeneration.

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