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A simple and cost-effective protocol for DNA isolation from buccal epithelial cells
Marisi Aidar1, Sergio Roberto Peres Line
1Department of Morphology, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
Brazilian Dental Journal
|November 6, 2007
Summary
This study introduces a fast, solvent-free method for extracting DNA from mouthwash samples. The DNA is stable for 30 days at room temperature, suitable for PCR amplification in epidemiological research.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Buccal cells are a valuable DNA source for epidemiological studies.
- Existing DNA extraction methods can be complex or require organic solvents.
Purpose of the Study:
- To develop a simple, solvent-free method for DNA extraction from mouthwash.
- To assess the stability of DNA in mouthwash solutions over time.
Main Methods:
- Utilized salting out for protein precipitation with saturated ammonium acetate.
- Avoided organic solvents in the DNA extraction protocol.
- Evaluated DNA yield and suitability for Polymerase Chain Reaction (PCR) amplification.
Main Results:
- The protocol is fast, economical, and allows simultaneous processing of multiple samples.
- DNA yield ranged from 5 to 93 microg (median 15 microg).
- Extracted DNA remained stable and suitable for PCR amplification after 30 days of room temperature storage.
Conclusions:
- This mouthwash-based DNA extraction method is efficient and convenient for epidemiological studies.
- The DNA's stability at room temperature simplifies sample handling and storage.
- The protocol's sensitivity and cost-effectiveness make it widely applicable.
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