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Published on: September 25, 2016
Controllable self-assembly from fibrinogen-gold (fibrinogen-Au) and thrombin-silver (thrombin-Ag) nanoparticle
Shibsekhar Roy1, Anjan Kumar Dasgupta
1Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700 019, India.
Abstract:
Fibrinogen conjugated gold nanoparticles (fibrinogen-Au) and thrombin conjugated silver nanoparticles (thrombin-Ag) were synthesized by heating (90 degrees C) the proteins (50 microg protein/ml) with 1mM AgNO(3) or AuCl(3). The resultant particles were harvested and examined by flow cytometry, scanning electron microscopy (SEM), transmission emission microscopy (TEM), optical microscopy and dynamic light scattering. SEM and TEM images revealed that the fibrinogen-Au and thrombin-Ag particles interacted. The emergent bio-nanoconjugate population could be controlled by addition of thrombin-Ag. The method may be exploited in parametrizing coagulation factors and other clinically important protein-protein interactions.

