Application of reverse micelle extraction process for amylase recovery using response surface methodology

Area of Science:

• Biochemistry
• Chemical Engineering
• Enzyme Technology

Background:

• Amylase enzymes are crucial in various industrial applications.
• Efficient recovery of amylase is essential for cost-effective production.
• Reverse micelle extraction offers a promising method for enzyme recovery.

Purpose of the Study:

• To investigate and optimize the reverse micelle extraction process for amylase.
• To determine the significant process variables affecting amylase recovery.
• To establish optimal conditions for maximizing amylase yield.

Main Methods:

• Solid-state fermentation was used for amylase production.
• Response surface methodology (RSM) with central composite rotatable design (CCRD) was employed.
• Analysis of process variables including pH, surfactant (AOT) concentration, and KCl concentration.

Main Results:

• AOT concentration and KCl concentration were identified as significant factors influencing amylase recovery.
• pH was found to be non-significant in the studied range.
• Optimal conditions for maximum enzyme recovery were determined as pH 10.43, 0.05 M AOT, and 1.00 M KCl.

Conclusions:

• The study successfully optimized the reverse micelle extraction of amylase.
• The identified optimal conditions achieved a high enzyme recovery of 83.16%.
• This optimization provides a valuable protocol for industrial amylase recovery.