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Updated: Jul 10, 2026
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Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Published on: October 3, 2018
DNA fragmentation based combinatorial approaches to soluble protein expression Part II: library expression, screening
Renos Savva1, Chrisostomos Prodromou, Paul C Driscoll
1Institute of Structural Molecular Biology, School of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, United Kingdom. p.driscoll@ucl.ac.uk
Drug Discovery Today
|November 13, 2007
Summary
This review explores expression screening of DNA fragment libraries for identifying stable, soluble protein domains. It details methods for screening DNA fragment populations and assessing expressed protein quality for protein domain hunting.
Area of Science:
- Molecular Biology
- Protein Chemistry
- Biotechnology
Background:
- Identifying soluble protein domains is crucial for structural and functional studies.
- Random, combinatorial methods offer a powerful approach to domain hunting.
- Expression screening of DNA fragment libraries is a key technique in this field.
Purpose of the Study:
- To review strategies for expression screening of DNA fragment libraries to identify soluble protein domains.
- To summarize methods for assessing the quality and reliability of expressed protein fragments.
- To provide an overview of the application of these techniques in protein domain hunting.
Main Methods:
- Construction of domain length-encoding DNA fragment libraries in expression vectors.
- Screening of these libraries to identify DNA fragments expressing stable, soluble protein fragments.
- Evaluation of various strategies for expression screening and protein quality assessment.
Main Results:
- A variety of alternative strategies for identifying stable protein truncates have been employed.
- Measures to determine the quality of expressed proteins and their reliability are discussed.
- The application extent of these measures within featured studies is analyzed.
Conclusions:
- Effective screening of DNA fragment libraries is essential for successful protein domain hunting.
- Reliable methods for assessing protein quality are critical for downstream applications.
- This review provides a comprehensive summary of current approaches in expression screening for protein domain identification.
