Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Transport Chain: Complex III and IV

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...

Bicarbonate-Carbonic Acid Buffer

Bicarbonate-Carbonic Acid Buffer

The carbonic acid-bicarbonate buffer system is critical for maintaining the body's pH balance. It operates on the equilibrium:

Carbon-dioxide Fixation

Carbon-dioxide Fixation

Carbon dioxide fixation in prokaryotes enables the assimilation of inorganic carbon into organic molecules, supporting biosynthetic pathways, sustaining ecosystems, and contributing to the global carbon cycle. It also has industrial applications in carbon capture and bioproduct synthesis. Autotrophic organisms rely on this process to utilize CO₂ as a carbon source in diverse environments.The Calvin CycleThe Calvin cycle is the most widespread carbon fixation mechanism, primarily used by...

The Supercomplexes in the Crista Membrane

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...

Bacterial Phylum Cyanobacteria

Bacterial Phylum Cyanobacteria

Cyanobacteria are a diverse group of oxygenic, phototrophic bacteria that played a pivotal role in converting Earth’s atmosphere from anoxic to oxygen-rich billions of years ago. They exhibit remarkable morphological diversity, ranging from unicellular forms to filamentous types, with cell sizes varying between 0.5 μm and 100 μm. Cyanobacteria are classified into five groups: Chroococcales (unicellular, dividing by binary fission), Pleurocapsales (unicellular, dividing by multiple fission),...

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterizing the Protein Isoforms of <i>foraging</i> (<i>for</i>), the PKGI Ortholog in <i>Drosophila melanogaster</i>.

International journal of molecular sciences·2023

Same author

Characterization of the C-terminal extension of carboxysomal carbonic anhydrase from Synechocystis sp. PCC6803.

Functional plant biology : FPB·2020

Same author

Inorganic carbon acquisition and its energization in eustigmatophyte algae.

Functional plant biology : FPB·2020

Same author

Evidence for convergent sensing of multiple abiotic stresses in cyanobacteria.

Biochimica et biophysica acta. General subjects·2019

Same author

Paper-based platform for detection by hybridization using intrinsically labeled fluorescent oligonucleotide probes on quantum dots.

The Analyst·2018

Same author

A thylakoid-located carbonic anhydrase regulates CO<sub>2</sub> uptake in the cyanobacterium Synechocystis sp. PCC 6803.

The New phytologist·2018

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Published on: November 10, 2021

A multiprotein bicarbonate dehydration complex essential to carboxysome function in cyanobacteria.

Swan S-W Cot¹, Anthony K-C So, George S Espie

¹Department of Biology, University of Toronto, Mississauga, Mississauga, Ontario L5L 1C6, Canada.

Journal of Bacteriology

|November 13, 2007

Summary

Researchers identified a protein complex within cyanobacterial carboxysomes that facilitates carbon dioxide concentration for photosynthesis. This complex, the bicarbonate dehydration complex (BDC), integrates key enzymes like carbonic anhydrase (CcaA) into the carboxysome shell via CcmM proteins.

More Related Videos

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Published on: May 27, 2019

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Related Experiment Videos

Last Updated: Jul 10, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Published on: November 10, 2021

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Looking Outwards: Isolation of Cyanobacterial Released Carbohydrate Polymers and Proteins

Published on: May 27, 2019

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

Published on: October 15, 2019

Area of Science:

Biochemistry
Molecular Biology
Cyanobacterial Physiology

Background:

Carboxysomes are essential protein compartments in cyanobacteria, crucial for the CO2-concentrating mechanism.
These organelles concentrate inorganic carbon to enhance the efficiency of photosynthesis by supplying CO2 to Rubisco.
The precise assembly and functional integration of proteins within carboxysomes remain incompletely understood.

Purpose of the Study:

To elucidate the composition and assembly of functional protein complexes within Synechocystis sp. strain PCC6803 carboxysomes.
To identify the interactions mediating the association of catalytic proteins with the carboxysome shell.
To understand the role of CcmM proteins in organizing the bicarbonate dehydration complex (BDC).

Main Methods:

Biochemical activity assays to measure enzymatic function.
Molecular approaches including yeast two-hybrid analysis to determine protein-protein interactions.
Analysis of protein complexes associated with isolated carboxysome shells.

Main Results:

A multi-protein bicarbonate dehydration complex (BDC) was identified, associated with the carboxysome shell.
The BDC minimally comprises CcmM73, CcaA, and CcmN, with larger complexes including Rubisco and other CcmM forms.
CcmM73 mediates shell association via interactions with CcmK/CcmL and recruits CcaA through its N-terminal domain, positioning CcaA as the catalytic core.

Conclusions:

CcmM proteins are central to organizing the BDC and integrating catalytic enzymes into the carboxysome structure.
The specific interaction between CcmM73 and CcaA is crucial for BDC assembly and function.
CcmM73 may act as a regulatory component of the BDC, binding bicarbonate without catalyzing its dehydration.