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Related Experiment Videos

Trehalose transport in yeast cells.

J H Crowe1, A D Panek, L M Crowe

  • 1Department of Zoology, University of California, Davis 95616.

Biochemistry International
|July 1, 1991
PubMed
Summary

Saccharomyces cerevisiae has a specific trehalose transporter. Glucose levels regulate this transporter, with transport increasing after glucose depletion and decreasing upon glucose addition, requiring protein synthesis for recovery.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Yeast Physiology

Background:

  • Trehalose is a crucial storage carbohydrate in Saccharomyces cerevisiae, providing stress resistance.
  • Understanding trehalose transport is key to comprehending yeast's metabolic regulation and adaptation.
  • Previous studies have indicated the presence of trehalose transport but lacked detailed characterization.

Purpose of the Study:

  • To investigate the regulation of trehalose transport in Saccharomyces cerevisiae.
  • To determine the role of glucose in modulating trehalose uptake.
  • To differentiate the trehalose transporter from other sugar transporters, such as the maltose transporter.

Main Methods:

  • Monitoring trehalose transport rates under varying glucose conditions (presence, absence, re-addition).
  • Assessing the time-dependent changes in transport activity.
  • Investigating the requirement of protein synthesis for the recovery of transport.
  • Comparing trehalose transport with maltose transport kinetics.

Main Results:

  • Trehalose transport is low during the exponential growth phase and increases significantly after glucose exhaustion in the stationary phase.
  • Addition of glucose to stationary phase cells rapidly inhibits trehalose transport.
  • Recovery of trehalose transport after glucose removal is dependent on new protein synthesis and exhibits a lag phase.
  • Kinetic analysis suggests that the trehalose transporter is distinct from the maltose transporter.

Conclusions:

  • Trehalose transport in Saccharomyces cerevisiae is tightly regulated by glucose availability.
  • The regulation mechanism involves glucose-mediated inhibition and requires protein synthesis for reactivation.
  • A specific transporter for trehalose exists, separate from the maltose transporter, highlighting specialized sugar uptake systems in yeast.

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