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Effect of monocrotophos on mono amine oxidase activity in albino rats
K V Swamy1, T Srinivas, P M Mohan
1Department of Zoology, Sri Venkateswara University, Tirupati, India.
Abstract:
Monocrotophos inhibited monoamine oxidase (MAO) activity both in vitro and in vivo in liver, kidney and brain areas of albino rats. In vitro effect was more pronounced than the in vivo effect. During in vivo daily treatment with sublethal doses of Monocrotophos, the MAO activity was significantly inhibited after 1h to 7 days of treatments. Later recovery of inhibition was noticed which might be attributed to the enhanced absorption of Monocrotophos to protein and fat bodies or enhanced metabolic dispositional mechanisms. Brain areas exhibit varied responses to Monocrotophos toxicity.
Insights
Monocrotophos, an insecticide, inhibits monoamine oxidase (MAO) activity in rats. This inhibition occurs in vitro and in vivo, with effects varying across organs and showing signs of recovery over time.
Area of Science:
- Toxicology
- Biochemistry
- Neuroscience
Background:
- Monoamine oxidase (MAO) is a critical enzyme in neurotransmitter metabolism.
- Organophosphate pesticides, like Monocrotophos, are known neurotoxicants.
- Understanding Monocrotophos's impact on MAO is vital for assessing its toxicity.
Purpose of the Study:
- To investigate the in vitro and in vivo effects of Monocrotophos on MAO activity.
- To determine the time course of MAO inhibition and subsequent recovery following Monocrotophos exposure.
- To explore regional differences in brain MAO response to Monocrotophos.
Main Methods:
- In vitro enzyme assays to measure MAO activity.
- In vivo administration of Monocrotophos to albino rats.
- Analysis of MAO activity in liver, kidney, and various brain regions at different time points.
Main Results:
- Monocrotophos significantly inhibited MAO activity both in vitro and in vivo.
- In vitro inhibition was more potent than in vivo inhibition.
- MAO activity was reduced within 1 hour of in vivo treatment, with recovery observed over longer periods.
- Brain regions displayed differential sensitivity to Monocrotophos-induced MAO inhibition.
Conclusions:
- Monocrotophos is a potent inhibitor of MAO, impacting key organs and brain regions.
- The observed recovery suggests adaptive mechanisms or altered Monocrotophos disposition.
- Varied brain responses highlight the complexity of Monocrotophos neurotoxicity.
