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Updated: Jul 10, 2026
Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
Platelet gel stimulates proliferation of human dermal fibroblasts in vitro
M Krasna1, D Domanović, A Tomsic
1Department for Blood Supply, Blood Transfusion Center of Slovenia, Slajmerjeva 6, 1000 Ljubljana, Slovenia. metka.krasna@ztm.si
Background:
Platelet gel is a source of many soluble proteins that can modulate tissue regeneration. Dermal fibroblasts play an important role in tissue remodeling and wound healing.
Objective:
The study was performed to investigate the influence of platelet gel and its active ingredients on the proliferation of human dermal fibroblasts in vitro.
Methods:
A fibroblast culture was established from a normal human skin biopsy. Platelet gel was prepared from platelet-rich plasma (PRP) following the addition of calcified thrombin solution. Cell proliferation was measured by MTT assay.
Results:
We showed that platelet gel, PRP, and thrombin stimulate the proliferation of dermal fibroblasts, and that stimulation by PRP is dose dependent.
Conclusion:
Platelet gel can be used to treat chronic skin defects.
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