Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiplexed detection methods for profiling microRNA expression in biological samples.

Alastair W Wark1, Hye Jin Lee, Robert M Corn

  • 1Department of Chemistry, University of California-Irvine, Irvine, CA 92697, USA.

Angewandte Chemie (International Ed. in English)
|November 13, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aerosol flow-driven instability and droplet-interfacial stabilization of mRNA lipid nanoparticles during mesh nebulization.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

A Single CeO<sub>2</sub>/Polydopamine/Multiwalled Carbon Nanotube Hybrid Sensing Platform for Concurrent Voltammetric Monitoring of Benzo[a]pyrene, Pyrene, and Fluorene in Particulate Matter.

ACS sensors·2026
Same author

Recent Advances in Nanoscale Sprayable Hydrogels for Cancer Management: Nanoparticle-Loaded Formulations for Therapy and Prevention.

International journal of nanomedicine·2026
Same author

Chromium nitride nanozyme-enhanced lateral flow immunoassay for sensitive and selective detection of transglutaminase 2: Proof-of-concept toward liver cancer-related biomarker evaluation.

Biosensors & bioelectronics·2026
Same author

Effects of Oral Health and Functional Characteristics on Taste Sensitivity in Older Adults: Comparative Analysis Using Solution and Taste Strip Tests.

Journal of oral rehabilitation·2026
Same author

Differential microRNA Expression Profiles in Girls with Idiopathic Central Precocious Puberty and Premature Thelarche.

International journal of molecular sciences·2026
Same journal

Fundamentals, Measurement and Regulation of the Conductance of Single Molecule Junctions.

Angewandte Chemie (International ed. in English)·2026
Same journal

Quantitative Photoswitching of Spin States in o-Fluoroazobenzene-Loaded Metal-Organic Frameworks.

Angewandte Chemie (International ed. in English)·2026
Same journal

Cobalt Nanoparticles Confined in Defective Carbon Matrices for Robust Intermittent CO<sub>2</sub> Methanation.

Angewandte Chemie (International ed. in English)·2026
Same journal

Copper(II/III) Redox Couple Enables C─H Methylation via a Radical Mechanism Analogous to SAM Enzymes.

Angewandte Chemie (International ed. in English)·2026
Same journal

Ring Strain Engineering of Cyclic Ethers for High-Performance Sodium Metal Batteries.

Angewandte Chemie (International ed. in English)·2026
Same journal

Bond Length as a Unified Descriptor for Stable Iodine Battery.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Short RNA molecules called microRNAs (miRNAs) regulate gene expression, adding complexity to cell function. New high-throughput methods are needed for rapid, multiplexed detection of these crucial molecules in biological samples.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Discovery of short, non-protein coding RNA molecules, like microRNA molecules (miRNAs), has revealed complex gene expression regulation.
  • Hundreds to thousands of miRNAs are expressed in plants and animals, driving significant research interest since 2001.
  • Understanding miRNA regulatory roles is crucial for cell function and biological processes.

Purpose of the Study:

  • To review the latest quantitative bioanalytical methods for high-throughput miRNA profiling.
  • To highlight the technological challenges in the rapid, multiplexed detection of miRNAs.
  • To provide an overview of advancements in miRNA detection technologies.

Main Methods:

  • Discussion of various high-throughput miRNA profiling techniques.

Related Experiment Videos

  • Analysis of methods enabling rapid and multiplexed detection.
  • Focus on quantitative bioanalytical approaches.

    • Main Results:

    • Identification of key technological challenges in miRNA detection.
    • Overview of current state-of-the-art methods for miRNA profiling.
    • Emphasis on the need for improved sensitivity and throughput.

    Conclusions:

    • Advancements in high-throughput miRNA profiling are essential for biological research.
    • Overcoming technological hurdles is critical for comprehensive miRNA analysis.
    • Accurate and efficient miRNA detection will deepen our understanding of gene regulation.