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Updated: Jul 10, 2026

Characterization of Biological Absorption Spectra Spanning the Visible to the Short-Wave Infrared
Published on: January 10, 2025
Differential optical spectroscopy for absorption characterization of mono & two-layered scattering media
Cyril Billet1, Raphael Sablong
1CREATIS-LRMN, CNRS UMR 5220, Université de Lyon, France. Cyril.Billet@univ-lyon1.fr
Abstract:
Diffuse reflectance techniques are commonly used to characterize the optical properties of various biological tissues in the visible and NIR spectral ranges. Nevertheless a sharp determination of local chromophores concentration in turbid media is usually difficult to obtain because of the nonlinear dependence of the reflected light intensity as a function of scattering and absorption coefficients. The proposed technique of this paper allows to determine absorbent compounds concentration ratios in a turbid medium from three reflectance spectra, measured for source-detector distances inferior to 1 cm. This method is validated in the visible range in case of typical biological absorption and reduced scattering coefficients. Two-layered scattering media are also investigated.
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