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Related Concept Videos

Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
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Published on: April 6, 2022

Sterilization using 365 nm UV-LED.

Noriyuki Yagi1, Mirei Mori, Akiko Hamamoto

  • 1Institute of Technology and Science, The University of Tokushima.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|November 16, 2007
PubMed
Summary

Ultraviolet light-emitting diodes (UV-LEDs) offer an eco-friendly sterilization method. This study demonstrates their effectiveness in eliminating E. coli and Vibrio parahaemolyticus, suggesting their potential as a novel sterilization device.

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Area of Science:

  • Microbiology
  • Environmental Science
  • Biotechnology

Background:

  • Traditional sterilization methods like chlorine and heat have limitations.
  • Ultraviolet (UV) sterilization is recognized for preserving object quality and environmental friendliness.
  • UV-LED technology presents advantages such as lacking harmful substances and extended operational lifespan.

Purpose of the Study:

  • To investigate the sterilization efficacy of UV-LEDs.
  • To evaluate the effectiveness of UV-LEDs against specific bacterial strains.
  • To assess the potential of UV-LEDs as a modern sterilization device.

Main Methods:

  • Utilizing UV-LEDs as the primary sterilization tool.
  • Exposing bacterial cultures (E. coli and Vibrio parahaemolyticus) to UV-LED irradiation.
  • Documenting the exposure durations required for complete germicidal effect.

Main Results:

  • Complete germicidal effects were observed for E. coli after 30 minutes of UV-LED exposure.
  • Complete germicidal effects were observed for Vibrio parahaemolyticus after 10 minutes of UV-LED exposure.
  • The results confirm the significant sterilization capabilities of UV-LEDs.

Conclusions:

  • UV-LEDs demonstrate potent germicidal effects against tested bacteria.
  • UV-LED technology is a viable and effective alternative for sterilization applications.
  • The findings support the use of UV-LEDs as a sustainable sterilization device.