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Related Experiment Video

Updated: Jul 10, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Microfluidic liquid filters for leukocyte isolation.

Jeffrey SooHoo1, Glenn Walker

  • 1Joint Department of Biomedical Engineering, University of North Carolina Chapel Hill and North Carolina State University, Raleigh, NC, USA.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
|November 16, 2007
PubMed
Summary

This study introduces a microfluidic device for gentle leukocyte separation from whole blood. It efficiently isolates white blood cells using a novel lysis and separation technique for sensitive downstream analysis.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Cell Separation

Background:

  • Accurate leukocyte quantification and rare cell detection require efficient and gentle cell separation methods.
  • Existing methods for isolating leukocytes from whole blood can be harsh, leading to cell damage and inaccurate results.

Purpose of the Study:

  • To develop and validate a microfluidic device for the efficient and gentle separation of leukocytes from whole blood.
  • To minimize leukocyte exposure to toxic lysing agents and remove non-leukocyte blood components.

Main Methods:

  • A microfluidic device utilizing an Aqueous Two Phase System (ATPS) with dextran (DEX) and polyethylene glycol (PEG) was employed.
  • Whole blood flowed in a DEX stream adjacent to a PEG stream containing a lysing solution, enabling selective erythrocyte lysis.

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Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
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Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

Published on: June 7, 2018

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Last Updated: Jul 10, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
07:37

Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

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  • Leukocytes were retained by surface tension while lysed cell remnants and other components were channeled away.

    • Main Results:

    • The device achieved efficient separation of leukocytes from whole blood.
    • Minimization of leukocyte exposure to the lysing solution was demonstrated.
    • Effective removal of lysed erythrocytes and other blood components was achieved.

    Conclusions:

    • The developed microfluidic device offers a gentle and efficient method for leukocyte separation.
    • This technique is suitable as a preparatory step for accurate leukocyte quantification and sensitive detection of rare leukocytes.