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Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
Multiple pathways differentially regulate global oxidative stress responses in fission yeast
Dongrong Chen1, Caroline R M Wilkinson, Stephen Watt
1Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom.
Molecular Biology of the Cell
|November 16, 2007
Summary
Cellular protection from oxidative stress involves distinct regulatory pathways in yeast. Different oxidants activate specific gene expression programs, revealing pathway plasticity and backup mechanisms for cellular defense.
Area of Science:
- * Molecular biology
- * Cellular stress response
- * Yeast genetics
Background:
- * Cellular protection against oxidative damage is crucial for aging and disease.
- * Understanding gene expression regulation under oxidative stress is key.
Purpose of the Study:
- * To analyze genome-wide gene expression programs and their regulation in response to various oxidants in *Schizosaccharomyces pombe*.
- * To identify key regulatory pathways and factors involved in oxidative stress response.
Main Methods:
- * Genome-wide gene expression analysis using transcriptomics.
- * Genetic manipulation of regulatory factors (Pap1p, Prr1p, Sty1p, Atf1p, Hsr1p, Pmk1p).
- * Exposure to different types and doses of oxidants (hydrogen peroxide, t-butylhydroperoxide).
Main Results:
- * A core set of genes regulated by Pap1p and Prr1p was universally induced.
- * Stronger oxidative stress triggered broader transcriptional responses involving MAPK Sty1p and bZIP factor Atf1p, particularly for hydrogen peroxide.
- * A novel zinc-finger protein, Hsr1p, globally supported gene expression under hydrogen peroxide stress.
- * The response to t-butylhydroperoxide showed differential regulation, involving MAPK Pmk1p, with less reliance on Sty1p-Atf1p.
Conclusions:
- * *Schizosaccharomyces pombe* exhibits significant plasticity in its oxidative stress response pathways.
- * Distinct regulatory mechanisms provide specificity and backup for cellular protection against different oxidants.
- * The findings contribute to understanding the complex interplay of cellular defense systems against oxidative damage.
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