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Related Experiment Videos

Flow cytometric assays for quantifying activated ovine platelets.

Carl A Johnson1, Trevor A Snyder, Joshua R Woolley

  • 1Bioengineering Department, University of Pittsburgh, PA, USA.

Artificial Organs
|November 17, 2007
PubMed
Summary
This summary is machine-generated.

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New assays can now detect activated ovine platelets, improving biocompatibility testing for cardiovascular devices in sheep. This enhances preclinical evaluation of artificial organs and device design.

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Device Development
  • Preclinical Research

Background:

  • Ovines are crucial animal models for evaluating cardiovascular devices like artificial organs.
  • Assessing device biocompatibility in ovines is vital but lacks sufficient tools.
  • Current methods for detecting ovine platelet activation are limited.

Purpose of the Study:

  • To develop and validate assays for quantifying ovine platelet activation.
  • To evaluate the cross-reactivity of anti-human platelet antibodies and annexin V in ovine models.
  • To enhance biocompatibility assessment in ovine preclinical studies.

Main Methods:

  • Incubation of ovine whole blood with candidate markers (anti-human CD62P antibodies, annexin V).
  • Quantification of platelet binding to markers using flow cytometry.

Related Experiment Videos

  • Development of an assay for quantifying platelet microaggregates.

    • Main Results:

    • Several anti-human CD62P antibodies and annexin V demonstrated selective binding to activated ovine platelets.
    • The developed assays successfully quantified ovine platelet activation.
    • The study identified reliable markers for ovine platelet activation.

    Conclusions:

    • Validated assays for ovine platelet activation improve biocompatibility data quality in preclinical studies.
    • These tools support the evaluation of cardiovascular devices in the ovine model.
    • Enhanced assessment aids in refining artificial organ design for better biocompatibility.