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Updated: Jul 10, 2026
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
Published on: April 3, 2018
[Construction of tissue engineered gingiva with gingival epithelia and acellular dermal matrix]
Chang-yun Fang1, Xiao-li Xie, Bin-jie Liu
1Department of Stomatology, Xiangya Hospital, Changsha 410008, China. cyfangdental@mail.csu.edu.cn.
Objective:
To culture human gingival epithelia in vitro, and to construct the tissue engineered gingiva with the acellular dermal matrix (ADM).
Methods:
Human gingival epithelia were isolated from the gingival tissue, and the cells were cultured and seeded onto the surface of ADM. After 7 days of submerged incubation, an air-liquid interface culture was performed for 7, 14, and 21 days. The complex constructed above was taken for histological examination.
Results:
Human gingival epithelia could proliferate well on the surface of ADM, and form multilayer structure. But the superficial epithelium was partially keratinized.
Conclusion:
Tissue engineered gingiva may be constructed with human gingival epithelia and ADM in vitro.
