Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Holographic tomographic volumetric additive manufacturing.

Nature communications·2025
Same author

HoloTile light engine: new digital holographic modalities and applications.

Reports on progress in physics. Physical Society (Great Britain)·2024
Same author

Phase-only steerable photonic nanojets.

Optics express·2023
Same author

Optical catapulting of microspheres in mucus models-toward overcoming the mucus biobarrier.

Journal of biomedical optics·2019
Same author

Light-driven micro-tool equipped with a syringe function.

Light, science & applications·2018
Same author

Natural convection induced by an optically fabricated and actuated microtool with a thermoplasmonic disk.

Optics letters·2018

Related Experiment Video

Updated: Jul 10, 2026

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
08:48

Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

Published on: October 13, 2011

Multiple-beam optical tweezers generated by the generalized phase-contrast method.

René L Eriksen, Paul C Mogensen, Jesper Glückstad

    Optics Letters
    |November 17, 2007
    PubMed
    Summary
    This summary is machine-generated.

    Researchers applied the generalized phase-contrast (GPC) method to create a multiple-beam optical tweezer system. This system successfully trapped and held four 1-micrometer polystyrene beads using a phase mask and GPC technology.

    More Related Videos

    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
    06:53

    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

    Published on: November 18, 2022

    Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
    09:31

    Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

    Published on: May 27, 2013

    Related Experiment Videos

    Last Updated: Jul 10, 2026

    Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
    08:48

    Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers

    Published on: October 13, 2011

    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
    06:53

    Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

    Published on: November 18, 2022

    Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
    09:31

    Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

    Published on: May 27, 2013

    Area of Science:

    • Optics and Photonics
    • Biophysics
    • Microscopy

    Background:

    • Optical tweezers are essential tools for manipulating microscopic particles.
    • Developing multi-beam optical tweezers enhances manipulation capabilities.
    • Generalized phase-contrast (GPC) offers advanced imaging and manipulation techniques.

    Purpose of the Study:

    • To demonstrate the integration of the generalized phase-contrast (GPC) method with optical tweezer systems.
    • To implement a multiple-beam optical tweezer setup using GPC.
    • To validate the system's ability to trap and manipulate microparticles.

    Main Methods:

    • Utilized a fixed phase mask in conjunction with a GPC system.
    • Configured a multiple-beam optical tweezer system.
    • Employed polystyrene beads (1-micrometer diameter) as test particles in solution.

    Main Results:

    • Successfully generated four distinct optical tweezers.
    • Demonstrated the stable trapping and holding of 1-micrometer polystyrene beads.
    • Validated the efficacy of the GPC method in a multi-tweezer configuration.

    Conclusions:

    • The generalized phase-contrast (GPC) method is effectively applicable to multiple-beam optical tweezer systems.
    • This GPC-based system provides a robust platform for precise manipulation of microparticles.
    • The study confirms the potential for advanced optical manipulation using GPC technology.