Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multicellular sprouting during vasculogenesis.

Andras Czirok1, Evan A Zamir, Andras Szabo

  • 1Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, Kansas 66160, USA.

Current Topics in Developmental Biology
|November 21, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Decoding the Apical-Basal Surfaceome of Colon Epithelial Cells via Side-Selective Biotinylation.

Biomolecules·2026
Same author

Molecular dynamics simulations elucidate the misfolding mechanisms of secretion-defective pancreatic lipase variants.

The FEBS journal·2026
Same author

[Physician, painter, soldier: Georges Chicotot (1855-1937)].

Orvosi hetilap·2026
Same author

Fermented and Unfermented Rooibos (<i>Aspalathus linearis</i>) Exhibit Selective Protection Against Hepatic Stress in Rats Exposed to Fumonisin B1.

Antioxidants (Basel, Switzerland)·2026
Same author

Effects of Dietary Supplementation with Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Frass on Common Carp (<i>Cyprinus carpio</i>).

Animals : an open access journal from MDPI·2026
Same author

Multidisciplinary aspects of pediatric cochlear implantation: anesthesiological considerations.

Cochlear implants international·2026
Same journal

Building a resilient ovarian reserve: Early soma-oocyte interactions.

Current topics in developmental biology·2026
Same journal

Role of macrophages in testis function.

Current topics in developmental biology·2026
Same journal

Role of retinoic acid in meiosis.

Current topics in developmental biology·2026
Same journal

Impact of cancer immunotherapies on oocyte health and ovarian function.

Current topics in developmental biology·2026
Same journal

The ovarian stroma as a key regulator of follicular development and gamete quality across the reproductive lifespan.

Current topics in developmental biology·2026
Same journal

Intercellular cyclic nucleotide dynamics mediate oocyte meiosis in mammalian preovulatory follicles.

Current topics in developmental biology·2026
See all related articles

Multicellular organisms form complex patterns through cell and extracellular matrix interactions. A new model explains vascular plexus formation via cell adhesion and sprouting, mimicking natural patterns without complex signaling.

Area of Science:

  • Developmental biology
  • Biophysics
  • Cell biology

Background:

  • Living organisms exhibit complex multicellular patterns.
  • Automatized microscopy enables time-resolved tracking of embryonic development at cellular resolution.
  • Recent studies distinguish tissue deformation from cell-autonomous motion using ECM displacements.

Purpose of the Study:

  • To investigate the mechanisms underlying the formation of the primary vascular plexus.
  • To understand the roles of cell-cell and cell-extracellular matrix (ECM) interactions in vascular patterning.
  • To develop a model explaining multicellular sprout formation.

Main Methods:

  • Utilizing automatized microscopy for time-resolved tracking of embryonic development.
  • Analyzing extracellular matrix (ECM) displacements to differentiate tissue deformation and cell motion.

Related Experiment Videos

  • Developing an interacting particle model to simulate vascular plexus formation.

    • Main Results:

    • The polygonal vascular structure arises from cell-cell and cell-ECM interactions, including adhesion and sprouting.
    • Multicellular sprouts invade avascular areas via avb3 integrins and elongate through endothelial cell supply and migration.
    • An interacting particle model successfully replicates robust sprouting dynamics and patterns similar to native vascular plexuses.
    • The model explains sprout formation through preferential attraction to elongated structures, without requiring chemotaxis or mechanochemical signaling.

    Conclusions:

    • Vascular plexus patterning is driven by collective endothelial cell actions, specifically adhesion and sprouting.
    • A general mechanism based on attraction to elongated structures explains the prevalence of multicellular sprouting.
    • The developed interacting particle model provides a robust explanation for vascular pattern formation consistent with experimental observations.