Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multilocus sequence typing (MLST) of Staphylococcus aureus.

Nicholas A Saunders1, Anne Holmes

  • 1Communicable Disease Microbiology Services Support Division, Centre for Infections, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phylogenetic relationship and virulence composition of <i>Escherichia coli</i> O26:H11 cattle and human strain collections in Scotland; 2002-2020.

Frontiers in microbiology·2023
Same author

Prevalence of Shiga Toxin-Producing <i>Escherichia coli</i> O157 in Wild Scottish Deer with High Human Pathogenic Potential.

Animals : an open access journal from MDPI·2023
Same author

Analysis of <i>Escherichia coli</i> O157 strains in cattle and humans between Scotland and England & Wales: implications for human health.

Microbial genomics·2023
Same author

Resolution of Melanoma to Programmed Death-1 Blockade but Simultaneous Rapid Progression of Concomitant Chronic Lymphocytic Leukemia.

Acta haematologica·2022
Same author

Shiga toxin-producing <i>Escherichia coli</i> clonal complex 32, including serotype O145:H28, in the UK and Ireland.

Journal of medical microbiology·2022
Same author

Sustained expression of HPV16 E7 oncoprotein promotes p-AKT(Ser473)/p-Src(Tyr527) signaling to drive precancerous lesions to invasive cervical cancer.

Carcinogenesis·2022
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Multilocus sequence typing (MLST) provides detailed insights into the global spread of bacteria like Staphylococcus aureus. High-quality sequence data and curated web tools ensure the reliability and accessibility of this DNA-based typing method.

Area of Science:

  • Microbiology
  • Genetics
  • Bioinformatics

Background:

  • Multilocus sequence typing (MLST) is a standardized DNA-based method for bacterial strain characterization.
  • It analyzes conserved housekeeping genes to identify distinct genetic profiles.

Purpose of the Study:

  • To highlight the utility of MLST for understanding the global dissemination of Staphylococcus aureus.
  • To emphasize the importance of data quality and available web tools for MLST analysis.

Main Methods:

  • MLST involves sequencing specific conserved genes encoding essential proteins.
  • Sequence data is analyzed to assign a unique allelic profile (ST) to each isolate.

Main Results:

  • MLST provides sufficient discriminatory power to map the global population structure of Staphylococcus aureus.

Related Experiment Videos

  • The method is adaptable regarding sequence acquisition but requires high-quality data for accurate typing.

    • Conclusions:

    • MLST is a robust and widely accepted tool for epidemiological surveillance and population genetics studies.
    • Well-maintained web-based resources facilitate the global application and data analysis of MLST schemes.